The Portugal forward died along with his younger brother Andre Silva in the accident in Zamora in the early hours of Thursday morning.
News of Jota's death has sent shockwaves throughout football, with tributes flooding in from across the world.
"Liverpool Football Club are devastated by the tragic passing of Diogo Jota," his club said in a statement.
"The club have been informed the 28-year-old has passed away following a road traffic accident in Spain along with his brother, Andre.
"Liverpool FC will be making no further comment at this time and request the privacy of Diogo and Andre's family, friends, team-mates and club staff is respected as they try to come to terms with an unimaginable loss.
"We will continue to provide them with our full support."
Liverpool have opened a book of condolence, both physical in the Anfield Road Stand and online, lowered flags to half-mast and closed all stores and the museum and suspended all tours until Monday.
"Heartbroken" former Liverpool manager Jurgen Klopp, who signed Jota from Wolves in 2020, admits he is struggling to comprehend Jota's death.Klopp wrote on Instagram: ""I'm heartbroken to hear about the passing of Diogo and his brother Andre. Diogo was not only a fantastic player, but also a great friend, a loving and caring husband and father! We will miss you so much!"
Current head coach Arne Slot insists everyone associated with the club owes it to Jota to "stand together and be there for one another".
Slot revealed they were in "absolute shock" and said on the club's website: "What to say? What can anyone say at a time like this when the shock and the pain is so incredibly raw?
"I wish I had the words but I know I do not.
"All I have are feelings that I know so many people will share about a person and a player we loved dearly and a family we care so much about.£
Former Liverpool captain Steven Gerrard posted: "RIP Diogo. Condolences to his family and friends during this incredibly sad time."
The English Premier League said "football has lost a champion who will be forever missed."
Women's Euro 2025 teams observed a minute of silence ahead of group matches in Switzerland on Thursday to commemorate Jota and his brother, Andre
Players from Belgium and Italy, and later from Spain and Portugal, stood in silence before the games in Sion and Bern, respectively.
Meanwhile, fans left wreaths, scarves and shirts at a makeshift shrine outside Anfield. There were also similar scenes outside his previous club Wolves' ground, Molineux.
The accident came less than two weeks after Jota married his long-term partner Rute Cardoso. The couple have three children together.
Local police in Spain say they are still investigating the accident in Spain.
International team-mate Cristiano Ronaldo wrote on X: "It doesn't make sense. Just now we were together in the National Team, just now you had gotten married.
"To your family, your wife, and your children, I send my condolences and wish them all the strength in the world. I know you will always be with them. Rest in Peace, Diogo and Andre. We will all miss you."
Jota won 49 caps for Portugal, scoring 14 international goals, and last month won the Nations League for a second time.
He scored 65 goals in 182 appearances for Liverpool, with nine of those goals coming last season's Premier League win.
He signed for Wolves, initially on loan, from Atletico Madrid in the summer of 2017 and helped Wolves win the Championship in his first season.
Jota began his career with Portuguese side Pacos de Ferreira before moving to Atletico, where he did not play a game but was loaned out to Porto.
His brother spent time in Porto's youth team before playing senior football for Gondomar and second-tier side Penafiel.
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