ANZ executives will give evidence at the Senate inquiry into increasing closures of country branches at a hearing in Ingham, Queensland, on Thursday.
Committee chair Senator Matt Canavan wrote to all banks in February asking them to halt closures during the inquiry in an act of good faith.
ANZ said it would postpone announcements of further shut downs, but would go ahead with 14 closures planned last year.
"We reviewed whether we could pause these processes. On balance, we believe doing this would be more disruptive to our customers and staff than completing them as planned," ANZ's response said.
Commonwealth Bank and Westpac agreed to Senator Canavan's request, while NAB said it would continue with a "reshaping" process that included closures.
The committee has heard bank closures leave rural communities fearing for the future of their towns, which rely on face-to-face services for farming operations and access to cash for events and volunteer organisations.
Banks say the closures reflect a shift towards digital banking and a decline in foot traffic.
The inquiry sat in Cloncurry, in north west Queensland, on Wednesday, a community that successfully halted the closure of its Westpac branch.
Mayor Greg Campbell said locals showed executives the bank was leaving town during a major economic boom, including new mining and energy projects worth at least $9 billion.
"It just seemed to make no sense at all, from a business perspective," Mr Campbell said.
"They've seen, hopefully, they won't be able to make a decision like that quietly in this town."
Janessa Bidgood, the president of the Curry Merry Muster, said the branch was critical to the festival in dealing with large sums of cash.
The event usually attracts about 6000 people over four days and has a $500,000 turnover, she said.
"We have built it to a point where we would consider ourselves a significant customer of Westpac.
"And yet we weren't awarded the same respect as other customers."
After the closure of a supermarket, some Cloncurry locals have been doing their food shopping 120km away in Mount Isa and the loss of a bank would have the same affect, Ms Bidgood said.
"It hurts little communities like Cloncurry in more ways than just 'you can't get to the bank'.
"It's not Westpac's responsibility, but this is why we fought so hard for little things to stay open.
"We need a food shop, we need a bank, we need a hairdresser, we need a butcher, we need those things in our town so that people don't go to another town."