Over the next four years, the Labor government will spend $945.7 million to reduce pest populations, minimise biosecurity risks to livestock, and lower the risk of environmental damage.
NSW Agriculture Minister Tara Moriarty said the biosecurity plan encompasses everything from feral pig management to tackling weeds and pests on public lands and fighting red fire ants.
"Strong biosecurity protections are not just about keeping pests and weeds out," she said in a statement on Friday.
"It is about protecting the state's $22 billion agriculture industries and ensuring families across the state have access to the world'ss best produce at the world's best prices."
The state's bus network is also set for an upgrade, with a $139.5 million boost to modernise the ageing fleet.
The upgrade work, due to begin later this year, will involve 4000 Opal-enabled buses in Greater Sydney and another 1000 in outer metropolitan areas.
Sydney's buses are also in line for a technology and service upgrade in the new financial year. (Dan Himbrechts/AAP PHOTOS)
The work includes new on-board screens and audio facilities to keep commuters informed and the elimination of so-called "ghost buses" via new technology to provide live and accurate service information, the government said.
More services, new routes and more zero-emission buses are also on the agenda.
Communities in western Sydney - where some suburbs still lack essential transport services - are set to benefit the most from the changes.
Those in Sydney's northwest will see an increase in the frequency and service hours on many existing routes, as well as more bus connections to train and metro stations.
Transport Minister Jo Haylen said transport developments have not kept up with population growth in the city's west.
"Millions of people rely on buses - they're the cornerstone of a thriving, interconnected and reliable public transport network," she said.
NSW Treasurer Daniel Mookhey will hand down the budget for 2024/25 on Tuesday.