Demonstrators will meet at the State Library of Victoria at midday on Sunday to call for an end to the war in Gaza.
Other pro-Palestine rallies are planned for Sydney's Hyde Park and Brisbane's King George Square across the day.
People are also expected to gather in Sydney in support of Israel.
It comes after pro-Palestine and pro-Israel groups faced off in Melbourne on Friday night following a suspicious fire at a nearby Palestinian-owned burger shop.
Police used pepper spray as a fight broke out in the middle of Hawthorn Road in Caulfield South and one person was hit by a rock.
Victoria Police Inspector Scott Dwyer said he was confident the fire was not religiously or politically motivated but security footage showed two people setting the shop alight and fleeing in a car parked nearby.
Burgertory owner Hash Tayeh, who has agreed to speak at Sunday's Melbourne rally, believes the fire was a hate crime sparked by his involvement in a pro-Palestine rally.
Friday night's clash prompted the evacuation of a nearby synagogue, which the protest organisers have apologised for.
Police have stepped up patrols in the Caulfield area in response.
Prime Minister Anthony Albanese was among those to condemn the scenes, saying all Australians have a responsibility to preserve peace.
"There is no place in our nation for hatred or prejudice of any kind," he said.
Federal Opposition Leader Peter Dutton hit out at what he described as "a deliberate act of incitement designed to end in violence" and anti-semitism.
Palestinian academic Micaela Sahhar and Victorian Greens Leader Samantha Ratnam are also slated to address Sunday's rally in Melbourne.
4°C