Peter Addo, 19, Abel Sorzor, 19, and Prince Conteh, 20, all formally entered not guilty pleas in Melbourne Magistrates Court on Friday afternoon.
They are accused of attacking Chol Achiek, 12, and Dau Akueng, 15, at Cobblebank in Melbourne's outer northwest on September 6.
The two boys were on their way home from basketball when a group of males set upon them and stabbed them to death.
Five other accused - all aged 15 and 16 - have also been charged with two counts of murder.
At a preliminary hearing in April, the court was told police found a sword at one of the 16-year-old accused's homes after the alleged murders.
Preliminary testing suggested it was likely the 16-year-old boy's DNA was on the sword's handle, while the DNA of one of the slain boys was on the blade, the court was told.
Addo, Sorzor and Conteh each elected to have their cases fast-tracked to the Victorian Supreme Court where they will stand trial on two counts of murder.
Magistrate Stephen Ballek remanded them each into custody to face their first directions hearing on May 29.
Senior crown prosecutor Kristie Churchill SC also indicated the underage accused were planning to have their cases fast-tracked.
The five teens are expected to complete that process at a children's court next week.
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