Traffic in Brisbane was thrown into chaos on Tuesday as Blockade Australia also launched a simultaneous protest in Melbourne, blocking Webb Dock Road in front of the Port of Melbourne gates.
Queensland Police were alerted to a man who suspended himself over Port of Brisbane Rd about 6.20am, blocking all eastbound lanes.
The male protester refused orders to lower himself, and thousands of motorists were stranded as traffic ground to a halt.
The man was taken into custody and the road reopened in both directions as investigations continue.
It is the second straight day of protests after activists targeted ports in Newcastle, Brisbane and Melbourne on the anniversary of a high-profile raid, resulting in multiple arrests.
Three people have been charged over their involvement in the Monday protests, which were live-streamed to social media.
Monday's port protests were held on the same date that a rural property in Colo, northwest of Sydney, was raided last year.
Governments in Victoria, NSW, Tasmania and South Australia have recently moved to clamp down on climate protesters, passing legislation to steeply increase jail time and fines for disrupting major roads and facilities.
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