Curator Marcus Lobbes and his team were greeted with boos and whistles as they took to the stage at the end of Saturday evening's performance of "Götterdämmerung", German news agency dpa reported.
However, there was warm applause for the singers and musicians, and particularly for conductor Christian Thielemann.
Festival organisers said ahead of the performance that it would be the first time AI took part on stage at the event, "not as a character, but as an image-generating force".
They said singers would be "the fixed point within a visually seething cosmos of light, texture, history, and association, amidst projections that erupt, constantly shift, and merge into one another".
The AI-assisted projections included scenes from previous performances of Wagner's Ring cycle and historical themes, and drew from images pre-selected by Lobbes, the director of the Academy for Theater and Digitality in Dortmund, and his team.
The collage that the technology projected on stage puzzled the audience, dpa reported, with images including former German Chancellor Helmut Kohl, the towers of the World Trade Center after the September 11, 2001 attacks, and a special postage stamp marking German reunification in 1990.
The festival website said that "every performance will be unique - because the images and associations never stand still".
Two more performances are scheduled.
