New licence conditions will also require sellers to provide information about their e-cigarette suppliers, importers or manufacturers to allow the products to be traced.
The new measures come into force on July 10 with SA Health officers to conduct random visits to shops over the next two months.
The new conditions are an interim measure, while the federal government works through plans to stop the importation of non-prescription e-cigarettes and the banning of vapes in retail settings and single-use disposable vapes, announced last month.
"These strict new conditions are designed to keep illegal nicotine vapes out of retail outlets and out of the hands of South Australians, especially children," Health Minister Chris Picton said.
"Many e-cigarettes, particularly the disposable variety, are not labelled as containing nicotine when in fact they do contain nicotine, often in very high concentrations."
Previous enforcement blitzes over the past year resulted in the seizure of about 15,000 illegal nicotine vapes with 17 fines issued and three licences cancelled.
Under SA's new rules retailers must obtain written proof from suppliers that their vaping products are nicotine-free or arrange laboratory testing if their supplier cannot provide proper documentation.
Any breach of the rules could result in a fine of up to $10,000.
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